副猪嗜血杆菌potD基因缺失株的构建及其部分生物学特性

doi:10.11843/j.issn.0366-6964.2016.11.015

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (11): 2274-2279.doi: 10.11843/j.issn.0366-6964.2016.11.015

副猪嗜血杆菌potD基因缺失株的构建及其部分生物学特性

谨瑾¹，张禄滑¹，文心田¹，李英¹，代科¹，周鹏^1,2，赵玉佳¹，曹三杰¹，黄小波¹，伍锐¹，赵勤¹，文翼平^1*

（1．四川农业大学动物医学院猪病研究中心，成都 611130；2．四川省攀枝花市农牧局，攀枝花 617000）

收稿日期:2016-06-27 出版日期:2016-11-23 发布日期:2016-11-23
通讯作者: 文翼平，博士，E-mail：yueliang5189@163.com
作者简介:谨瑾(1993-)，女，山西运城人，硕士，主要从事动物传染病的研究工作，E-mail：JIN109JIN@163.com；张禄滑（1986-），男，四川内江人，博士，主要从事动物传染病的研究工作，E-mail：zhangluhua520@126.com。二人同为第一作者
基金资助:
公益性行业（农业）科研专项（201303034）

Construction and Characterization of a Haemophilus parasuis potD Mutant Strain

JIN Jin¹, ZHANG Lu-hua¹, WEN Xin-tian¹,LI Ying¹, DAI Ke¹, ZHOU Peng ^1,2, ZHAO Yu-jia¹, CAO San-jie¹, HUANG Xiao-bo¹, WU Rui¹, ZHAO Qin¹, WEN Yi-ping^1*

(1.Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; 2. Panzhihua Bureau of Agriculture and Animal Husbandry, Panzhihua 617000, China)

Received:2016-06-27 Online:2016-11-23 Published:2016-11-23

摘要/Abstract

摘要：

potD基因编码的蛋白质作为转运体蛋白，负责多胺的结合和转运，为细胞的正常生长提供必需因子，已在多种病原菌中鉴定为毒力相关因子，但在副猪嗜血杆菌中的作用还不清楚。利用自然转化法构建potD缺失株SC1401ΔpotD::kan，再比较亲本株和缺失株的生长特性、自凝集活性、生物被膜形成能力、抗血清杀菌能力以及对小鼠的致病力。结果显示potD基因的缺失不影响副猪嗜血杆菌的生长、自凝集活性和生物被膜形成能力，但导致该菌的抗血清杀菌能力和对小鼠的致病力显著降低。以上结果表明potD基因可能与副猪嗜血杆菌的毒力相关。

Abstract:

As a transporter, protein encoded by potD is involved in the binding and transportation of polyamine, which is necessary for the growth of cells. In some pathogens, the protein is identified as a virulence-associated factor, but of which the function is still unclear in Haemophilus parasuis. A potD mutant strain SC1401ΔpotD::kan was constructed by the natural transformation method. The growth curve, the ability of autoagglutination and biofilm formation, serum resistance ability and the virulence to mice of the parental strain SC1401 and potD mutant strain were measured. No significant difference was observed between the parental and mutant strains in the growth curve and the ability of autoagglutination and biofilm formation. However the potD mutant strain showed an obvious decrease in serum resistance ability and the virulence to mice. The findings above suggested that potD gene may be associated with virulence in H. parasuis.

中图分类号:

S852.613

谨瑾，张禄滑，文心田，李英，代科，周鹏，赵玉佳，曹三杰，黄小波，伍锐，赵勤，文翼平. 副猪嗜血杆菌potD基因缺失株的构建及其部分生物学特性[J]. 畜牧兽医学报, 2016, 47(11): 2274-2279.

JIN Jin, ZHANG Lu-hua, WEN Xin-tian,LI Ying, DAI Ke, ZHOU Peng, ZHAO Yu-jia, CAO San-jie, HUANG Xiao-bo, WU Rui, ZHAO Qin, WEN Yi-ping. Construction and Characterization of a Haemophilus parasuis potD Mutant Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2274-2279.

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参考文献

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副猪嗜血杆菌potD基因缺失株的构建及其部分生物学特性

Construction and Characterization of a Haemophilus parasuis potD Mutant Strain

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